ERYTHROPOIETIC CELL CULTURES FROM CHICK EMBRYOS
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چکیده
منابع مشابه
Erythropoietic Cell Cultures from Chick Embryos
Erythropoietic cell cultures from very early chick blastoderms survive for several days They show four to seven doublings of the erythroid cells and the appropriate morphological changes from proerythroblasts to mature erythrocytes Cell cycle times are the same as in ovo for the first day of culture, but slow down thereafter The hemoglobins of both the primitive and the definitive red cell seri...
متن کاملCulture of Erythropoietic Cells from Chick Blastoderms
Erythropoiet ic cells from early chick embryos have been successfully ma in ta ined in vitro bo th in tissue culture (Murray, 1932; Settle, 1954; O'Brien, 1960; Spra t t and Haas, 1960 a and b; Hell, 1964; Levere and Granick, 1967; Wilt , 1967; Hagopian and Ingram, 1971; and Wenk, 1971) as well as in suspension culture (Hagopian et al., 1972). In all cases potent ia l erythropoiet ic cells will...
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During studies on the fate of leprosy bacilli in the fibroblasts of lepromatous lesions (1), occasion was taken to utilize the bacilli from any extra portions of the nodules in trying to establish experimental infections in chick embryos and chick tissue cultures. Due to the two different types of experimental material, the literature, methods, and results pertaining to each will be summarized ...
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Here we describe a method for preparing and culturing primary cells dissociated from Drosophila gastrula embryos. In brief, a large amount of staged embryos from young and healthy flies are collected, sterilized, and then physically dissociated into a single cell suspension using a glass homogenizer. After being plated on culture plates or chamber slides at an appropriate density in culture med...
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Tissue culture cells of human origin (HeLa, Lich, and AV(3)) were inoculated with the AL complex. By immunofluorescent staining, AL complex antigen was detectable in the cytoplasm of infected cells as punctate fluorescent granules during the early stage and as homogeneous fluorescence during the late stage of infection. By combining fluorescence and phase-contrast microscopy, many infected cell...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1972
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.54.1.98